refractive index detector dri wyatt optilab trex Search Results


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Waters Corporation optilab t rex
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Viscotek Corporation ve 3210 uv vis detector
Ve 3210 Uv Vis Detector, supplied by Viscotek Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Mals System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Waters Corporation downstream multiangle light scattering detector
Dimerization of FlSp NT analyzed by SEC-MALS. Proteins purified at pH 5.5 and pH 8.0 were analyzed using a Superdex 75 increase SEC column equilibrated at each pH. The chromatograms show the differential refractive index (dRI, broken lines ) and light scattering signal (LS, solid lines ) at pH 5.5 ( red ) and pH 8.0 ( blue ) together with the molecular mass calculated by MALS at pH 5.5 ( orange ) and pH 8.0 ( cyan ). Representative measurements are shown for ( A ) WT FlSp NT, ( B ) FlSp NT∗, ( C ) FlSp NT E79QE84QE119Q , and ( D ) FlSp NT E79QE84QE119QE130Q . MALS data for FlSp NT E79QE84QE119QE130Q were associated with higher uncertainty (%). E , the experimentally determined average molecular masses were used to calculate the molecular weight ratio between pH 5.5 and pH 8.0 for each protein. FlSp, flagelliform spidroin; MALS, <t>multiangle</t> light scattering; NT, N-terminal; SEC, size-exclusion chromatography.
Downstream Multiangle Light Scattering Detector, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/refractive+index+detector+dri+wyatt+optilab+trex/miniDAWN/pmc09097459-202-6-36
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Dimerization of FlSp NT analyzed by SEC-MALS. Proteins purified at pH 5.5 and pH 8.0 were analyzed using a Superdex 75 increase SEC column equilibrated at each pH. The chromatograms show the differential refractive index (dRI, broken lines ) and light scattering signal (LS, solid lines ) at pH 5.5 ( red ) and pH 8.0 ( blue ) together with the molecular mass calculated by MALS at pH 5.5 ( orange ) and pH 8.0 ( cyan ). Representative measurements are shown for ( A ) WT FlSp NT, ( B ) FlSp NT∗, ( C ) FlSp NT E79QE84QE119Q , and ( D ) FlSp NT E79QE84QE119QE130Q . MALS data for FlSp NT E79QE84QE119QE130Q were associated with higher uncertainty (%). E , the experimentally determined average molecular masses were used to calculate the molecular weight ratio between pH 5.5 and pH 8.0 for each protein. FlSp, flagelliform spidroin; MALS, <t>multiangle</t> light scattering; NT, N-terminal; SEC, size-exclusion chromatography.
Ohpak Sb 806hq, supplied by Shodex, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GE Healthcare optilab rex refractive index detector wyatt
Dimerization of FlSp NT analyzed by SEC-MALS. Proteins purified at pH 5.5 and pH 8.0 were analyzed using a Superdex 75 increase SEC column equilibrated at each pH. The chromatograms show the differential refractive index (dRI, broken lines ) and light scattering signal (LS, solid lines ) at pH 5.5 ( red ) and pH 8.0 ( blue ) together with the molecular mass calculated by MALS at pH 5.5 ( orange ) and pH 8.0 ( cyan ). Representative measurements are shown for ( A ) WT FlSp NT, ( B ) FlSp NT∗, ( C ) FlSp NT E79QE84QE119Q , and ( D ) FlSp NT E79QE84QE119QE130Q . MALS data for FlSp NT E79QE84QE119QE130Q were associated with higher uncertainty (%). E , the experimentally determined average molecular masses were used to calculate the molecular weight ratio between pH 5.5 and pH 8.0 for each protein. FlSp, flagelliform spidroin; MALS, <t>multiangle</t> light scattering; NT, N-terminal; SEC, size-exclusion chromatography.
Optilab Rex Refractive Index Detector Wyatt, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Waters Corporation ri detector optilab rex
Dimerization of FlSp NT analyzed by SEC-MALS. Proteins purified at pH 5.5 and pH 8.0 were analyzed using a Superdex 75 increase SEC column equilibrated at each pH. The chromatograms show the differential refractive index (dRI, broken lines ) and light scattering signal (LS, solid lines ) at pH 5.5 ( red ) and pH 8.0 ( blue ) together with the molecular mass calculated by MALS at pH 5.5 ( orange ) and pH 8.0 ( cyan ). Representative measurements are shown for ( A ) WT FlSp NT, ( B ) FlSp NT∗, ( C ) FlSp NT E79QE84QE119Q , and ( D ) FlSp NT E79QE84QE119QE130Q . MALS data for FlSp NT E79QE84QE119QE130Q were associated with higher uncertainty (%). E , the experimentally determined average molecular masses were used to calculate the molecular weight ratio between pH 5.5 and pH 8.0 for each protein. FlSp, flagelliform spidroin; MALS, <t>multiangle</t> light scattering; NT, N-terminal; SEC, size-exclusion chromatography.
Ri Detector Optilab Rex, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Waters Corporation optilab t rex differential refractive index detector
Dimerization of FlSp NT analyzed by SEC-MALS. Proteins purified at pH 5.5 and pH 8.0 were analyzed using a Superdex 75 increase SEC column equilibrated at each pH. The chromatograms show the differential refractive index (dRI, broken lines ) and light scattering signal (LS, solid lines ) at pH 5.5 ( red ) and pH 8.0 ( blue ) together with the molecular mass calculated by MALS at pH 5.5 ( orange ) and pH 8.0 ( cyan ). Representative measurements are shown for ( A ) WT FlSp NT, ( B ) FlSp NT∗, ( C ) FlSp NT E79QE84QE119Q , and ( D ) FlSp NT E79QE84QE119QE130Q . MALS data for FlSp NT E79QE84QE119QE130Q were associated with higher uncertainty (%). E , the experimentally determined average molecular masses were used to calculate the molecular weight ratio between pH 5.5 and pH 8.0 for each protein. FlSp, flagelliform spidroin; MALS, <t>multiangle</t> light scattering; NT, N-terminal; SEC, size-exclusion chromatography.
Optilab T Rex Differential Refractive Index Detector, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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JASCO Inc optilab rex refractive index detector
Dimerization of FlSp NT analyzed by SEC-MALS. Proteins purified at pH 5.5 and pH 8.0 were analyzed using a Superdex 75 increase SEC column equilibrated at each pH. The chromatograms show the differential refractive index (dRI, broken lines ) and light scattering signal (LS, solid lines ) at pH 5.5 ( red ) and pH 8.0 ( blue ) together with the molecular mass calculated by MALS at pH 5.5 ( orange ) and pH 8.0 ( cyan ). Representative measurements are shown for ( A ) WT FlSp NT, ( B ) FlSp NT∗, ( C ) FlSp NT E79QE84QE119Q , and ( D ) FlSp NT E79QE84QE119QE130Q . MALS data for FlSp NT E79QE84QE119QE130Q were associated with higher uncertainty (%). E , the experimentally determined average molecular masses were used to calculate the molecular weight ratio between pH 5.5 and pH 8.0 for each protein. FlSp, flagelliform spidroin; MALS, <t>multiangle</t> light scattering; NT, N-terminal; SEC, size-exclusion chromatography.
Optilab Rex Refractive Index Detector, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Waters Corporation multi angle light scattering system
Dimerization of FlSp NT analyzed by SEC-MALS. Proteins purified at pH 5.5 and pH 8.0 were analyzed using a Superdex 75 increase SEC column equilibrated at each pH. The chromatograms show the differential refractive index (dRI, broken lines ) and light scattering signal (LS, solid lines ) at pH 5.5 ( red ) and pH 8.0 ( blue ) together with the molecular mass calculated by MALS at pH 5.5 ( orange ) and pH 8.0 ( cyan ). Representative measurements are shown for ( A ) WT FlSp NT, ( B ) FlSp NT∗, ( C ) FlSp NT E79QE84QE119Q , and ( D ) FlSp NT E79QE84QE119QE130Q . MALS data for FlSp NT E79QE84QE119QE130Q were associated with higher uncertainty (%). E , the experimentally determined average molecular masses were used to calculate the molecular weight ratio between pH 5.5 and pH 8.0 for each protein. FlSp, flagelliform spidroin; MALS, <t>multiangle</t> light scattering; NT, N-terminal; SEC, size-exclusion chromatography.
Multi Angle Light Scattering System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
Shodex kf 804 columns
Dimerization of FlSp NT analyzed by SEC-MALS. Proteins purified at pH 5.5 and pH 8.0 were analyzed using a Superdex 75 increase SEC column equilibrated at each pH. The chromatograms show the differential refractive index (dRI, broken lines ) and light scattering signal (LS, solid lines ) at pH 5.5 ( red ) and pH 8.0 ( blue ) together with the molecular mass calculated by MALS at pH 5.5 ( orange ) and pH 8.0 ( cyan ). Representative measurements are shown for ( A ) WT FlSp NT, ( B ) FlSp NT∗, ( C ) FlSp NT E79QE84QE119Q , and ( D ) FlSp NT E79QE84QE119QE130Q . MALS data for FlSp NT E79QE84QE119QE130Q were associated with higher uncertainty (%). E , the experimentally determined average molecular masses were used to calculate the molecular weight ratio between pH 5.5 and pH 8.0 for each protein. FlSp, flagelliform spidroin; MALS, <t>multiangle</t> light scattering; NT, N-terminal; SEC, size-exclusion chromatography.
Kf 804 Columns, supplied by Shodex, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Dimerization of FlSp NT analyzed by SEC-MALS. Proteins purified at pH 5.5 and pH 8.0 were analyzed using a Superdex 75 increase SEC column equilibrated at each pH. The chromatograms show the differential refractive index (dRI, broken lines ) and light scattering signal (LS, solid lines ) at pH 5.5 ( red ) and pH 8.0 ( blue ) together with the molecular mass calculated by MALS at pH 5.5 ( orange ) and pH 8.0 ( cyan ). Representative measurements are shown for ( A ) WT FlSp NT, ( B ) FlSp NT∗, ( C ) FlSp NT E79QE84QE119Q , and ( D ) FlSp NT E79QE84QE119QE130Q . MALS data for FlSp NT E79QE84QE119QE130Q were associated with higher uncertainty (%). E , the experimentally determined average molecular masses were used to calculate the molecular weight ratio between pH 5.5 and pH 8.0 for each protein. FlSp, flagelliform spidroin; MALS, multiangle light scattering; NT, N-terminal; SEC, size-exclusion chromatography.

Journal: The Journal of Biological Chemistry

Article Title: The dimerization mechanism of the N-terminal domain of spider silk proteins is conserved despite extensive sequence divergence

doi: 10.1016/j.jbc.2022.101913

Figure Lengend Snippet: Dimerization of FlSp NT analyzed by SEC-MALS. Proteins purified at pH 5.5 and pH 8.0 were analyzed using a Superdex 75 increase SEC column equilibrated at each pH. The chromatograms show the differential refractive index (dRI, broken lines ) and light scattering signal (LS, solid lines ) at pH 5.5 ( red ) and pH 8.0 ( blue ) together with the molecular mass calculated by MALS at pH 5.5 ( orange ) and pH 8.0 ( cyan ). Representative measurements are shown for ( A ) WT FlSp NT, ( B ) FlSp NT∗, ( C ) FlSp NT E79QE84QE119Q , and ( D ) FlSp NT E79QE84QE119QE130Q . MALS data for FlSp NT E79QE84QE119QE130Q were associated with higher uncertainty (%). E , the experimentally determined average molecular masses were used to calculate the molecular weight ratio between pH 5.5 and pH 8.0 for each protein. FlSp, flagelliform spidroin; MALS, multiangle light scattering; NT, N-terminal; SEC, size-exclusion chromatography.

Article Snippet: The column was connected to a downstream Multiangle Light Scattering Detector (miniDAWN TREOS, Wyatt, equipped with three MALS Detectors at the following angles: 43.63°, 90°, and 136.37°) and to a differential Refractive Index Detector (Optilab T-Rex, Wyatt).

Techniques: Purification, Refractive Index, Molecular Weight, Multi-Angle Light Scattering, Size-exclusion Chromatography